![]() Our Apoptosis Overview page goes into more detail about the two main apoptosis pathways. While it is not possible to measure intrinsically mediated apoptosis induction by flow cytometry, measuring extrinsically mediated apoptosis induction is relatively straightforward as it involves the analysis of the so-called death receptors (DR) CD95, CD261 (DR4), CD262 (DR5), CD120a, and CD120b, which can be detected using antibodies. To help you plan and optimize your flow cytometry experiments download or request a copy of our Flow Cytometry Basics Guide. We will also give you tips to avoid common pitfalls and help you choose the right assay. Here we will walk you through specific flow cytometry assays with examples that will enable you to detect apoptosis. Careful experimental set-up and interpretation of results will allow you to make the most of your experiment.įlow cytometry allows the study of all aspects of apoptosis from induction via surface receptors, to late stages where DNA fragmentation occurs. Other applications such as western blotting or imaging may need to be used. ![]() However, flow cytrometry may not be the most appropriate application. The powerful technique of flow cytometry can be used to both detect and quantify the level of apoptosis in a population of cells at static points or in a time course. For instance, if most of your cells are entering into late apoptosis you may not be able to detect early stages of apoptosis with some reagents. Before starting your experiments it is important to note that apoptosis is not a static process and certain tests are time specific. It offers the ability to study large numbers cells individually rather than a mixed population, examining simultaneously the expression of many proteins like cell specific markers and indicators of apoptosis. Continued abuse of our services will cause your IP address to be blocked indefinitely.Flow cytometry is one of the most popular and versatile applications for studying apoptosis. Please fill out the CAPTCHA below and then click the button to indicate that you agree to these terms. If you wish to be unblocked, you must agree that you will take immediate steps to rectify this issue. If you do not understand what is causing this behavior, please contact us here. If you promise to stop (by clicking the Agree button below), we'll unblock your connection for now, but we will immediately re-block it if we detect additional bad behavior. Overusing our search engine with a very large number of searches in a very short amount of time.Using a badly configured (or badly written) browser add-on for blocking content.Running a "scraper" or "downloader" program that either does not identify itself or uses fake headers to elude detection.Using a script or add-on that scans GameFAQs for box and screen images (such as an emulator front-end), while overloading our search engine.There is no official GameFAQs app, and we do not support nor have any contact with the makers of these unofficial apps. ![]() Continued use of these apps may cause your IP to be blocked indefinitely. This triggers our anti-spambot measures, which are designed to stop automated systems from flooding the site with traffic.
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